Methionine influences the profile of intestinal antibiotic resistome through inhibiting the growth of Escherichia coli
发布于: 2023-08-28 来源: 国家饲料工程技术研究中心
关键词：Methionine，Antibiotic resistance gene ，Sow intestine ，Microbial community
摘要：Antibiotic resistance genes (ARGs) are a new type of environmental pollutant. However, studies have mainly
focused on the distribution characteristics of ARGs in the livestock environment, lacking of studies on the
composition of ARGs in the intestinal tract of animals and the effect of nutrients on intestinal ARGs and microbial
communities. Reducing antimicrobial resistance and maintaining optimal animal health and performance are
urgently needed. Methionine is an essential amino acid which plays a critical role in the growth and reproductive
performance of animals. In this study, feeding experiment, in vitro fermentation and bacterial culture experiment
were performed to explore the influence of methionine on the intestinal resistome of sows. We found that dietary
0.2 % methionine supplementation decreased the total abundance of intestinal ARGs, which was further
confirmed by in vitro fecal microbial fermentation of sows. Metagenome binning analysis identified that
Escherichia coli was the major ARG host, which carried 60–113 ARGs and 134–286 virulence factors, indicating
that Escherichia coli in the pig intestine is not only a core ARG host, but also an important pathogen. In addition,
we found that methionine supplementation inhibited the growth of Escherichia coli, indicating that dietary
methionine may reduce the resistome risk in sow intestine by inhibiting core ARG hosts such as Escherichia coli.
These findings reveal that dietary methionine application plays a critical role in intestinal antibiotic resistance,
providing a new idea for preventing and controlling environmental pollution by antibiotic-resistant microbes.